Tempo-iMSC™
Human iPSC-derived Mesenchymal Stem Cells
Tempo-iMSC™
Human iPSC-derived Mesenchymal Stem Cells
Mesenchymal stem cells (“MSCs”) are non-haematopoietic, multipotent stromal stem cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, myocytes, and adipocytes. Tempo-iMSCTM are human iPSC-derived mesenchymal stem cells. Tempo-iMSCTM express cell surface markers such as CD73, CD90, CD166, and CD105, in addition to Oct4 and Nanog. This expression profile is predictive of the long-term proliferative capacity of cultured human MSCs (see reference: here). MSCs have immunomodulatory features, secrete cytokines and immune-receptors which regulate the microenvironment in the host tissue and they have multi-lineage potentials for a variety of cell types. MSCs can be used in a variety of screening applications, from screening biologics and small molecules to siRNAs. Due to MSCs’ ability to self-renew (= growth without differentiation), they are easily scalable for running large-scale assays.
NEW! For scientists working on exosomes, please email us for conditional media for exosome extractions and biomarker validations.
NEW! For scientists working on CD34+ HSCs, please email us regarding Tempo-iHSCTM (SKU214; Custom Requests Only).
Applications
Tempo-iMSC™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
Phenotypic Assays
High Content Imaging
Biomarker Discovery
Cytotoxicity Assays
Target Validation
Lead Optimization
Investigative Toxicology
Nonclinical Efficacy Evalutions
Live-cell Imaging
2D & 3D Cell Culture
Biomarker Authentication
Specifications
~1.25 x10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent monolayer
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
Technology used: in-house developed proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.
Starting Materials: human dermal/fibroblast cells
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)
Tempo-iMSC™ SKU209
Resources
Cell of the Month – Kupffer Cells
Welcome back to our Cell of the Month series! This time, we are talking about Kupffer cells - what they are, what they do, and why we should want to learn more about them! Kupffer cells - the gatekeepers of the liver First described by German...
The Forgotten Process – Trogocytosis!
Trogocytosis, from trogo meaning ‘to gnaw’ or ‘to nibble’ in Ancient Greek, is an active process during which one cell receives cell surface molecules and membrane fragments from another cell in a unidirectional and cell-contact dependent manner. ...
Tempo-iOligo™: Major study identifies TMEFF1 as a critical factor for HSV-1 replication in the central nervous system
Tempo-iOligo™was cited in Nature in a major infectious disease study that offers the first explanation as to why herpes simplex virus 1 (HSV-1) infection in the brain is very rare, despite the fact that most of us have been infected with the virus....