Tempo-iKer™
Human iPSC-derived Keratinocytes
Tempo-iKer™
Human iPSC-derived Keratinocytes
Keratinocytes are the predominant cells in the epidermal layer of human skin. ~90% of the human epidermis is constituted of keratinocytes. The most salient function of keratinocytes is their ability to form a barrier against outside damages by bacteria, fungi, parasites, viruses, heat, dehydration, and ultraviolet (UV).
Tempo-iKer™: human keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, non-viral, nucleic-acids-free, induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iKer™ cells are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratins 14 and 18. iKer™ can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.
Applications
Tempo-iKer™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
Phenotypic Assays
High Content Imaging
Biomarker Discovery
Cytotoxicity Assays
Target Validation
Lead Optimization
Investigative Toxicology
Nonclinical Efficacy Evalutions
Live-cell Imaging
2D & 3D Cell Culture
Biomarker Authentication
Specifications
~1.25×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)
Tempo-iKer™ SKU131