Human iPSC-derived Pericytes

Tempo-iPeri™

Human iPSC-derived Pericytes

In the human brain, cerebrovascular function is critical for CNS function. Pericytes belong to an important component of the neurovascular unit. Pericytes function as microcirculation cells and they are critical for the neurovascular unit (which includes brain microvascular endothelial cells, astrocytes and neurons). They communicate with endothelial cells of the small blood vessels and regulate the blood brain barrier. Dysfunctions or deficiencies in Pericytes lead to the breakdown of Blood Brain Barrier. Pericytes are integrators, coordinators and effectors of neurovascular functions including the regulation of the BBB permeability, regulation of cerebral blood flow and clearance of toxin.

Tempo-iPeri™ are human iPSC derived from nucleic-acids-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural-glial induction condition. Tempo’s iPeri™ cells are polarized compact cellular structures when plated as a monolayer in cell culture and express biomarkers such as CNPase and ZO-1 using (ICC) immunohistochemistry methods and additional biomarkers such as EDNRA, ENPEP, GRM8, KCNE4, NDUFA4L2, P2RY14, PLXDC1, RGS5, TRPC3, and PLXDC1 using RT/qPCR methods.